#Output cluster results with order and orientation information #Identify linear and circular topology in the contig graphĬluster_and_classify_GFA.pl humanHiC_100000_iced.matrix.100000.10.CtgContact.overCutoff.1.0.reciprocalMax.gfa > humanHiC_100000_iced.matrix.100000.10.CtgContact.overCutoff.1.0. #build contig graph by assigning links to contigs whose contact is larger than a given cutoff, and also satisfy reciprocal best requirement #adjust the contig contacts, and perform linear transformation, to find the turning point #calculate the HiC contact values among contigs, using Hi-C links data from fixed-size contig endsĬtgContact_from_ -binsize 100000 -binnum 10 hifiasm.fa.len humanHiC_100000_abs.bed humanHiC_100000.matrix > humanHiC_100000.matrix.100000.10.CtgContact Run endhic with specified contig end size and specified contact cutoff EndHiC programs: (the ranks shows invoking relationship) Generated by Hic-pro, 100-kb bins, normalized matrix fileĢ. Generated by Hic-pro, 100-kb bins, raw matrix file Generated by Hic-pro, 100-kb bins, bed format file Includes two column: contig_id contig_length Input files: (example, human hifiasm + hic-pro) After running HiC-pro, a recommended EndHiC usage for most users is to run with default parameters.ġ.
At Dynamic Design we can offer a bespoke methodology to ensure that the structure is constructed to the exact process in which it has been designed, reducing or eliminating entirely any potential risk to health and safety.EndHic is a fast and easy-to-use Hi-C scaffolding tool, using the Hi-C links from contig end regions instead of whole contig regions to assemble large contigs into chromosomal-level scaffolds.ĮndHiC takes the HiC-pro's bin matrix results as input data.
This is often required that for a complex structure, where it is important to either erect, modify or dismantle the structure in a specific sequence. We will identify all residual risks and clearly identify and state these within the design, which will enable you to manage these risks on site. Rest assured that if you require a site visit from us, you will get technical and practical advice from a design engineer with over 15 years in the scaffolding industry, who holds both advanced scaffolder and inspection cards.Ī designer’s risk assessment will be produced in accordance with the Construction, Design and Management Regulations 2015. We can also attend site to ascertain information for a future project or offer advice on the feasibility of the proposed work. Due to our experience and knowledge within the scaffold industry we are able to offer a wide range of on-site advice, whether this is carrying out a statutory 7-day scaffold inspection, or handover inspections.
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